Twins Roster & Staff
Transactions
| Date | Transaction |
| 03/25/25 | LHP Wilker Reyes assigned to Minnesota Twins. |
| 03/25/25 | RHP Jacob Wosinski assigned to Minnesota Twins. |
| 03/25/25 | RHP Eiberson Castellano returned to Philadelphia Phillies from Minnesota Twins. |
| 03/24/25 | Minnesota Twins optioned CF Austin Martin to St. Paul Saints. |
| 03/20/25 | 1B Javier Roman assigned to Minnesota Twins. |
| 03/18/25 | C Amilcar Vasquez assigned to Minnesota Twins. |
| 03/18/25 | C Amilcar Vasquez roster status changed by Minnesota Twins. |
| 03/18/25 | C Ricardo Pena assigned to Minnesota Twins. |
| 03/18/25 | SS Dameury Pena assigned to Minnesota Twins. |
| 03/18/25 | Minnesota Twins optioned C Jair Camargo to St. Paul Saints. |
| 03/17/25 | CF Byron Chourio assigned to Minnesota Twins. |
| 03/17/25 | Minnesota Twins released C Alex Isola. |
| 03/16/25 | Minnesota Twins optioned LHP Kody Funderburk to St. Paul Saints. |
| 03/16/25 | Minnesota Twins optioned RHP Zebby Matthews to St. Paul Saints. |
| 03/16/25 | Minnesota Twins optioned RHP David Festa to St. Paul Saints. |
| 03/14/25 | RHP Tyler Stasiowski assigned to Minnesota Twins. |
| 03/14/25 | RF Jayson Bass assigned to Minnesota Twins. |
| 03/14/25 | 2B Yohander Martinez assigned to Minnesota Twins. |
| 03/14/25 | Minnesota Twins released RHP Ezequiel Ventura. |
| 03/14/25 | LF Miguel Briceno assigned to Minnesota Twins. |
| 03/14/25 | RHP Michael Martinez assigned to Minnesota Twins. |
| 03/13/25 | C Jefferson Valladares assigned to Minnesota Twins. |
| 03/13/25 | LF Angel Del Rosario assigned to Minnesota Twins. |
| 03/13/25 | RHP Ivran Romero assigned to Minnesota Twins. |
| 03/13/25 | OF Caden Kendle assigned to Minnesota Twins. |
| 03/12/25 | Minnesota Twins optioned C Diego Cartaya to St. Paul Saints. |
| 03/11/25 | SS Brandon Winokur assigned to Minnesota Twins. |
| 03/11/25 | 3B Jorel Ortega assigned to Minnesota Twins. |
| 03/09/25 | Minnesota Twins optioned RHP Travis Adams to St. Paul Saints. |
| 03/09/25 | Minnesota Twins optioned RHP Matt Canterino to St. Paul Saints. |
| 03/09/25 | Minnesota Twins optioned CF Emmanuel Rodriguez to St. Paul Saints. |
| 03/09/25 | Minnesota Twins optioned RHP Marco Raya to St. Paul Saints. |
| 03/07/25 | Minnesota Twins signed free agent RHP Mitch Mueller to a minor league contract. |
| 03/07/25 | SS Ben Ross assigned to Minnesota Twins. |
| 03/06/25 | LF Kyler Fedko assigned to Minnesota Twins. |
| 03/06/25 | RHP Ezequiel Ventura assigned to Minnesota Twins. |
| 03/06/25 | 2B Tanner Schobel assigned to Minnesota Twins. |
| 03/06/25 | 3B Rayne Doncon assigned to Minnesota Twins. |
| 03/06/25 | RHP Juan Mendez assigned to Minnesota Twins. |
| 03/04/25 | LF Misael Urbina assigned to Minnesota Twins. |
| 03/02/25 | RHP Juan Mercedes assigned to Minnesota Twins. |
| 03/01/25 | RF Gabriel Gonzalez assigned to Minnesota Twins. |